- CODEN : IAJPBB
- issn 2349-7750
TITLE:
DEVELOPING A RAPID AND ECONOMICAL DNA EXTRACTION PROTOCOL FOR AN IMPORTANT MEDICINAL PLANT ARTEMISIA JUDAICA WITHOUT USING LIQUID NITROGEN.
AUTHORS:
Zahid Khorshid Abbas, Mohammad Nasir Khan
ABSTRACT:
Aims: Artemisia judaica in Saudi Arabia are exposed to serious threats due to heavy human impacts such as uncontrolled tourism, overgrazing and uncontrolled collection, mining, quarrying and natural drought. These activities warrant careful conservation strategies that would allow inclusion of these plants into development plans of the Saudi Arabia economy in order to ensure their sustainable use. The evaluation of the genetic diversity by using ISSR and RAPD PCR requires a high quality of intact DNA. Therefore the aim of this study was to establish a rapid DNA isolation protocol for Artemisia species without using Liquid Nitrogen. Results: In case of Artemisia species, the DNA protocol was optimized by using solution based method without using Liquid Nitrogen .The quality of DNA was checked on 0.7% agarose gel containing ethidium bromide at a final concentration of 0.5 mg/ml . This provides a very rapid and sensitive means of estimating the nucleic acid concentration. Similarly, the quantity and purity of DNA extracted from Artemisia species analyzed by using NanoDrop1000. The λ260/λ280 determined for Artemisia DNA samples were in a range of 1.83-2.0 and concentration (ug/ml) (502.7 to 1288.5 ng/µl) indicating a good quality DNA from our method. The protocol was simple, reliable and operative in normal laboratory condition in two hours. Conclusion: The developed protocol proved to be simple, reliable and operative in normal laboratory condition yielding intact DNA from Artemisia species having good quantity (170 to 1200 ng/µl) and quality (A260/280 ratio - 1.64 to 1.95) completed in two 2 to 3 hours . Key Words: Artemisia judaica , Liquid Nitrogen, DNA
FULL TEXT
Approved Journal
NLM ID:101711216 [Serial]
Included in EuroPub database
ICV 2015 - 61.16
Indexed In
S.J Impact Factor:7.157
cosmos Impact Factor:4.153
ICMJE Recommendations
IP Indexing Impact Factor: 2.76
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