- CODEN : IAJPBB
- issn 2349-7750
TITLE:
NOVEL SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF PHYCOCYANIN IN SPIRULINA: APPLICATION TO ASSAY METHOD VALIDATION
AUTHORS:
Devadasu Ch*, Srinivasa Babu P, Sowjanya S, Prasada Rao CV, Sony Ch, Rukhmini D, Rabbani Md, Radha M, Bhavyaprabha K
ABSTRACT:
A reliable spectrophotometric method has been developed for quantitative analysis of Phycocyanin in capsule formulations by developing a suitable extraction scheme for complete extraction of phycocyanin from its biomass with the help of central composite design and then the phycocyanin thus obtained was treated with diazotised sulphanilic acid as an active coupling species. The red-colored species generated on an ice-cold condition has shown absorption maximum at about 490 nm. The method was validated in that its linearity was excellent within the concentration range of 100-500 µg/mL with correlation coefficient 0.9998. The intra-day and inter-day precision were found as less than 2.0 % RSD and the recovery studies proved its accuracy as the % recovery is of 99.97-100.36. Commercially available capsule formulation was assayed and the mean % assay was found as 99.87%. KEYWORDS: Spirulina, Phycocyanin, sulphanilic acid, diazotised, Validation
FULL TEXT
Approved Journal
NLM ID:101711216 [Serial]
Included in EuroPub database
ICV 2015 - 61.16
Indexed In
S.J Impact Factor:7.157
cosmos Impact Factor:4.153
ICMJE Recommendations
IP Indexing Impact Factor: 2.76
Cover Page
