- CODEN : IAJPBB
- issn 2349-7750
TITLE:
DEVELOPING AMPLIFICATION REFRACTORY MUTATION SYSTEM –PCR SYSTEM FOR RAPID DETECTION OF CYP2C9*2 (RS1799853) C>T GENE VARIATION IN T2D PATIENTS OF TABUK-SAUDI ARABIA
AUTHORS:
Imadeldin Elfaki*, Rashid Mir, Fahad M, Almutairi
ABSTRACT:
Background: The CYP2C9 is a drug-metabolizing enzyme and the second expressed CYP isoforms in hepatocytes. Single nucleotide polymorphisms (SNPs) influence the catalytic activity of CYP2C9.The CYP2C9*2 (rs1799853) is an important SNP in CYP2C9 gene results in replacement of Arginine144 to Cysteine. This SNP has been reported to reduce the activity of CYP2C9. Therefore the aim of this study was to establish a rapid and sensitive molecular based assay for the detection of CYP2C9*2 (rs1799853) C>T gene variation in T2D patients. Methodology: This study was conducted on 25 clinically confirmed cases of T2D patients and 25 healthy controls. DNA extraction was performed with the Qiagen Kit. The amplification-refractory mutation system (ARMS)-PCR was optimized to detect CYP2C9*2 (rs1799853) *2 C>T gene variation. Results: This study was conducted on 50 samples (25 were T2D patients and 25 were healthy controls). ARMS –PCR was optimized to detect CYP2C9*2 (rs1799853) *2 C>T gene variation in T2D patients and healthy controls. Conclusion: ARMS-PCR system for detection of (rs1799853) gene variation was successfully optimized. The assay proved to be fast, accurate, simple and economical that does not require any special equipment other than a thermocycler. The ARMS-PCR system can be used as a potential molecular tool for the detection of CYP2C9 gene variations in T2Ds. Key Words Type 2 diabetes (T2D) - CYP2C9*2 (rs1799853) *2 C>T (Arg>Cys) - amplification-refractory mutation system (ARMS)-PCR
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NLM ID:101711216 [Serial]
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ICV 2015 - 61.16
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S.J Impact Factor:7.157
cosmos Impact Factor:4.153
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IP Indexing Impact Factor: 2.76
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