Dr Aaisha Qadir, Dr Sidra Khan, Dr Muhammad Usman Hasan
Objective: To evaluate the vitamin B 12 deficiency frequency in individuals with high mean corpuscular volume and anemia. Study design: A cross-sectional study. Place and Duration: In the Medicine Unit II of Services Hospital Lahore for one year duration from September 2017 to September 2018. Methods: 113 samples were included according to the exclusion and inclusion criteria. The deficiency of Vitamin B 12 in serum was determined according to standard methods. By Sysmex KX 21 hematology analyzer, Blood samples were analyzed and by Tukey-Cramer and ANOVA test continuous variables were analyzed and analysis of categorical variables was done by Chi-square test. The relationship among variables was determined by Pearson correlation. Statistically significant p ≤ 0.05 was obtained. Results: Of the 113 subjects, 37 (32.7%) were male, female were 76 (67.2%), 34.48 ± 6.71 years was the mean standard and anemic patients were 89.3% (n = 101). The mean hematocrit, red blood cells and hemoglobin were 3.0 million, dl, 33.1% / μL, 11.3 g respectively. Vitamin B12 deficiency was observed in 65 of 113 subjects (57.5%); In addition, 19.4% and 37.9% respectively are divided into sub-sections as limit and absolute deficiency. Mean corpuscular volumes of up to 139 fl and low levels of vitamin B12 up to <30 pg / ml were observed. In 11 (9.7%) patients¸ Pancytopenia was observed with severe deficiency of vitamin B 12 (<100 pg / ml). A strong negative association was noted between mean corpuscular volume and vitamin B 12 (p = 0.0001, r = -0.79). Poikilocytosis, anisocytosis, hypersegmented neutrophils and megaloblast were seen in peripheral blood film. Conclusion: Vitamin B 12 deficiency is an important factor contributing to mega-loblastic anemia. Key words: Megaloblastic anemia, Vitamin B 12 deficiency.