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TITLE:

DETERMINATION AND FINGERPRINTING OF GALLIC ACID IN HYDROALCOHOLIC EXTRACT OF HELICTERUS ISORA (MALVACEAE) BY HPTLC METHOD

AUTHORS:

Shruti S. Bhagwat and Padmanabh B. Deshpande* 8503-8508

ABSTRACT:

The medicinal plants are widely used by the traditional medicinal practitioners for curing various diseases. Helicteres isora plant is used traditionally for different activities like antimicrobial, antioxidant, anticancer, antiulcer, hepatoprotective, antineoplastic, antimicrobial, Anti-inflammatory. Phytochemical analysis of the plant confirmed the presence of carbohydrate, proteins, polyphenols, tannins, flavonoids, alkaloids, saponin, and steroid. In the current study, an effort was made to quantify the phenolic acid gallic acid in the hydroalcoholic extract of Helicteres isora. High performance thin layer chromatographic method has been used to determine the presence and to quantify gallic acid in Helicteres isora extract. Separation was carried out with the use of silica gel 60F254 precoated TLC plates as stationary phase and mixture of Chloroform: Ethyl acetate: Methanol: Formic acid (12:8:1.5:1.5, v/v/v/v) as mobile phase. Densitometric evaluation was carried out at 275 nm. A good linear relationship was obtained in the concentration range of 100-600 ng band-1. Keywords: Helicteres isora,, Gallic acid, HPTLC, Anti-inflammatory

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