Dr Syed Hassan Musanna, Dr Mashal Masud, Dr Rehan Munir
Objectives: We aimed in this analysis to observe the compassion of NS1 antimalaria ELISA examination of Dengue infection in patients. Study designed: A Case control study diagnosis Place and Duration: This study was conducted in the Microbiology Section, Department of Pathology, SIMS / Services Hospital, Lahore from January 2018 to December 2018. Methodology: This analysis consists of samplings of clinically examined patients who have Dengue infection according to their gender and age under the provision of their detailed information from day of fever. The blood samplings with number of 113 were collected by accurately labeled non-reusable syringes in consistence of fever days on request forms specifying the data of patients. Dengue NS1 antibody ELISA examinations were processed through an amber-colored, protein-rich liquid which separates out when blood coagulates on the current day. Samplings were obtained from male and female patients that were 64 and 49 in number. Patients having age more than 18 years were 84.0 % in percentage. They were analyzed with DF and DHF in the Emergency Department and were entitled in the Services Hospital Lahore. This analyzation continued for 1 year from January 2018 to December 2018. Blood samplings were obtained in the non-reusable injections by the SIMS Micro lab. The samplings of the blood were permitted to clot before the process where a mixture is separated through spinning at 4000 RPM for the dispersion of sera. NS1 antibody ELISA examination was processed. ELISA equipment that were developed by BIORD in France were processed for this. The examinations were processed in accordance to the guidelines advised by the developers. The enduring sera were kept in serum containers at temperature of 20.0 degree centigrade that were perfectly branded. Results: In the starting 6 days of contagion by virus of dengue there was a percentage of 50.0 % to 81.0 % optimistic in attitude of the NS1 antibody ELISA examination. Conclusions: By the first 7 days of contagion of dengue there was a maximum compassion found through NS1 antibody ELISA examination. So, it is advised that labs do not acquire advanced instruments and have minimum money for the PCR can process NS1 Antibody ELISA examination. Key Words: “day of fever”, Dengue hemorrhagic fever, Dengue NS1 antigen, Dengue Fever.