Ekramul Hasan, Rajbongshi Lata, Abdur Rouf , Howlader Saurav*
The aim of the present study was to evaluate the anti-diabetic, anti-inflammatory, and antioxidant potential of Heliotropium indicum leaf extracts. Anti-diabetic and anti-inflammatory activities were evaluated via protein tyrosine phosphatase (PTP1B) and rat lens aldose reductase inhibitory assays and cell-based lipopolysaccharide (LPS)-induced nitric oxide (NO) inhibitory assays in RAW 264.7 murine macrophages. In addition, scavenging assays using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical, and peroxynitrite (ONOO−) were used to evaluate antioxidant potential of Heliotropium indicum. The leaf extracts of Heliotropium indicum showed significant antioxidant effects in DPPH, ABTS, and ONOO− scavenging assays. Likewise, methanol (MeOH) extracts of H. indicum inhibited LPS-induced NO production in a dose dependent manner that was further clarified by suppression of iNOS and COX-2 protein production. The MeOH extracts of H. indicum exhibited potent inhibitory activities against PTP1B and RLAR with the corresponding IC50 values of 277.31±0.22 and 1561.32±3.48 µg/mL, respectively. Our study establishes that Heliotropium indicum extract might be useful as a potent anti-diabetic, antioxidant, and anti-inflammatory agent. Keywords: H. indicum; Protein tyrosine phosphatase 1B (PTP1B), Rat Lens Aldose Reductase (RLAR), Anti-diabetic, Anti-inflammation, Antioxidant.