Dr. Safia Moin, Dr. Azra Perveen, Dr. Uzma Yasmeen
Background: In order to identify the colonization standard of near-term pregnant women, many techniques have been employed. While on the most suitable technique, there is a shortage of consensus. Three different methods have been explained in this study and their sensitivity and specificity in performance is discussed. Objective: The study aimed at the assessment of the reliability of three laboratory techniques in the detection of group B streptococcus (Streptococcus Agalactias) (GBD) in near-term pregnant women. The laboratory techniques include direct culture on selective blood agar, direct culture on Islam agar and subculture after enrichment with Lim broth on Islam agar. Materials and Methods: Total 200 near-term pregnant females of all ages were included in the study which was organized at Allied Hospital, Faisalabad (October 2017 to May 2018). These women were in the age bracket of 35 – 37 weeks of gestation. For the assessment of swabs from vaginal introitus, they were cultures on 4 medians. A criterion was set on the basis of which reactivity and particularity of each media, were measured. Results: The reactivity and specificity of different methods were measured. The reactivity of blood agar, Islam Agar, and LIM enriched Agar was 93.3%, 98% and 100% respectively. The cases observed positive for GBS on given criteria were 32. Sixteen percent was the observed incidence. Conclusion: After LIM enrichment, the technique was better on the basis of its activity and cost is subculture on Islam Agar. Keywords: Agar, Islam Agar, LIM Agar, Blood Agar, Specificity, Sensitivity and Pre-Term.