Sabahat Anwar*, Humera Afrasiab, Naila Ali
In the present study, the objective was to evaluate the antioxidant and antimicrobial activities of methanolic extracts of different indigenous cultivars of garlic. The antioxidant activity of all the extracts was determined using two complementary methods, namely 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay and Hydrogen peroxide (H2O2) scavenging assay. All the cultivars exhibited antioxidant activity to different extent. Hazro cultivar indicated highest antioxidant activity both by DPPH assay and H2O2 scavenging assay as compared to other cultivars. Antimicrobial activity of the garlic extracts was also evaluated by using the agar dilution method against different microbial strains. The Pink local cultivar was most resistant to Staphylococcus aureus and Streptococcus viridians. while Desi cultivar was resistant against Escherichia coli and Clostridium septicum and Hazro cultivar was most resistant against Pasteurella multocida. Key words: Garlic, indigenous, methanolic extract, antioxidant activity, DPPH, H2O2, antimicrobial activity, Pakistan.