Iqra Zafar, Umm e Rubab, Umair Sohail Mehr
Introduction: Half of the world's population is still at risk of malaria. In 2008, an estimated 243 million cases were reported and nearly 863000 deaths were attributed to the disease. Aims and objectives: The basic aim of the study is to analyse the prevalence of human malaria infection in Pakistan. Material and methods: This cross-sectional study was conducted in Nishtar Medical University, Multan during January 2019 to December 2019. The data was collected from 200 male and female patients. Participants included in the study were under-5-year-old children, either admitted in the children's ward or attending any clinic on outpatient basis. Results: This study recruited 200 patients. Twelve patients were excluded due to incomplete data. 188 patients were tested using Paracheck-Pf RDT, while 167 patients were tested using thick-film microscopy. A total of 70 (41.9%) were found to be positive by microscopy and 62 (37.1%) by RDT. Thirty-four of the 70 positive results by microscopy were negative (false-negative rate of 48.6%), while 26 of the 97-negative thick-film microscopic results were positive (false-positive rate of 26.8%). Conclusion: It is concluded that interpreting test results without gold standard can be challenging. The use of RDTs in the diagnosis of malaria infection offers an easy-to-use, low-cost, and rapid testing.