Muhammad Mateen, Talha Irfan, Dr Muhammad Haseeb
Objective: The purpose of this study is to determine the association of the prolactin with prevalence of glucose intolerance in period of pregnancy. Methodology: This research work performed on 30 females with Gestational Diabetes Mellitus and 30 females with Impaired Glucose Tolerance and 30 healthy controls having fine glucose tolerance. All the participants were fasted overnight before test. We drew the sample of blood at 6.00 A.M. After that, 75.0 grams’ oral glucose dissolved in 200.0cc water was provided to every patient, waiting for 2 hours, we drew another sample of blood from each member. Fasting as well as two hours after 75.0 grams’ load of glucose, concentrations of plasma glucose (FBS & 2h-BS) were estimated by the method of glucose oxidase. We used immunoradiometric assay to measure the concentrations of insulin in fasting blood sample and sample after two hours with 75.0 grams’ glucose load sample. We also used specific radioimmunoassay for the measurement of concentrations serum prolactin in after two hours’ sample. Results: There was no important disparity in the level of fasting serum insulin of all participants of 3 groups (P>0.050) whereas, the average level of two hours’ serum insulin of group of females suffering from gestational diabetes mellitus group was much lower as compared to participants of impaired glucose tolerance and healthy controls groups (P <0.0050). Results showed that level of serum prolactin of healthy controls in 1st, 2nd and 3rd trimester increases with the increase in pregnancy period (P <0.00010). The findings of serum prolactin of control, gestational diabetes mellitus and impaired glucose tolerance groups in the last trimester stated that there was no significant difference in any two groups (P > 0.050. According to the findings, there highest average of the level of prolactin was present in the group of healthy controls. Conclusion: Level of prolactin increases with the increase of pregnancy period. This level reaches its peak value in the 3rd trimester when majority of the pregnant women may develop gestational diabetes mellitus because of condition of resistance to insulin. There are no proper evidences, if there is association of prolactin level with the insulin intolerance in females with pregnancy. Keywords: Gestational Diabetes Mellitus, Trimester, Pregnancy, Prolactin, Intolerance, Impaired Glucose Tolerance, Radioimmunoassay.