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EVALUATION OF IN VITRO ANTI-INFLAMMATORY AND ANTIOXIDANT POTENTIALS OF METHANOL LEAF EXTRACT OF FAGARA ZANTHOXYLOIDES

Enechi Osmund Chukwuma, Okeke Emmanuel Sunday*, Abonyi Uchenna Collins, Nwankwo Nicodemus Emeka, Ndimele Ugochi Joy, Benneth Moses Ebere

Background: Fagara zanthoxyloides is a medicinal plant used in traditional medicine for the treatment of elephantiasis, toothache, sexual impotence, malaria, dysmenorrheal and abdominal pain among rural dwellers. Objective: To study the In vitro anti-inflammatory and antioxidant activities of methanol extract of Fagara zanthoxyloides leaves. Materials and Methods: Methanol extract of Fagara zanthoxyloides leaves were used for this study. In vitro anti-inflammatory studies were performed for the extract using proteinase inhibition activity and albumin denaturation inhibition assays, while antioxidant activities were performed by determination of DPPH (1, 1-diphenyl-2-picrylhydrazyl) scavenging assay and hydrogen peroxide scavenging activity. Aspirin was used as a standard drug for the anti-inflammatory activity while vitamin C and E were used as standard drugs for the determinationof DPPH scavenging assay and hydrogen peroxide scavenging activity respectively. Results: At the concentration of 75 and 100 µg/ml, the methanol extract of Fagara zanthoxyloides showed significant (p < 0.05) inhibition of 39 and 44% of proteinase inhibitory action and, 42 and 63% of albumin denaturation inhibition activity. In addition, the extract had effective DPPH radical scavenging and hydrogen peroxide scavenging activities. At a concentration of 80 µg/ml, the extract showed an inhibition of 53 and 77% of DPPH radical scavenging and hydrogen peroxide scavenging activities respectively. Conclusion: The present study showed In vitro antioxidant and anti-inflammatory activities of the methanol extract of Fagara zanthoxyloides which scientifically prove the ethnomedicinal claims. Keywords: Fagara zanthoxyloides, anti-inflammatory, antioxidant, protease inhibition, albumin denaturation, DPPH , hydrogen peroxide.

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