ResearcherID - CLICK HERE Scientific Journal Impact Factor (SJIF-2020) - CLICK HERE

THE EFFECT OF PANAX GINSENG EXTRACT IN EXTENDER ON RAM SPERM PARAMETERS IN VITRO CONDITION

Saeed Safavi pour*, Akbar Pirestani , Fariborz Moattar

Sperm survived for a short time in undiluted semen, and semen slow cooling to a temperature of 5°C will cause the death of many spermatozoa. Thus, extender is suitable which sperm parameters preserved during cooling and freezing. The aim of this study was to investigate the effect of panax ginseng extract in extender on sperm motility, morphology and viability of Afshari rams. In this study, four Afshari rams with a mean of 5 ± 50 kg weight and 3 to 4 years, sperm was collected by electro ejaculation. After extraction of the medicinal plant at a concentration of 1%, 3% and 5%, each concentration was added to the sperm extender as separately and one group were considered as a control. Sperm motility, morphology and viability were evaluated at zero time (immediately post ejaculation) and 24 hours post ejaculation by CASA software, papanicolaou and Eosin-Nigrosin staining, respectively. The results of this study shows that progressive motility was significantly increased (p<0.05) in treatment groups of 3% and 1% panax ginseng extract compare to control and other treatment groups at time zero and 24 hour post ejaculation, respectively. However, normal sperm morphology was significantly higher (P<0.05) in treatment group of 3% extract than control and other treatment groups at zero time and 24 hour post ejaculation, respectively. Percentage of live sperm was significantly higher (P<0.05) in treatment group of 3% panax ginseng extract compare to other treatment groups at zero time and also, treatment group of 1% panax ginseng extract was significant higher viability (P<0.05) than to other treatment groups. Therefore, the addition of panax ginseng extract in extender was advisable to improve sperm parameters in afshari rams. Keywords: Ram Sperm, Panax Ginseng, Extender, Motility, Morphology, Viability.

FULL TEXT