Oral administration of drug dosage forms to treat dental problems yields slow action due to low onset of action and hepatic “first-pass.” To overcome these problems in this work, it was planned to prepared and characterize the In-Situ Gels for periodontal applications for effective treatment at the site of the action. The technique (Sol-Gel transformed systems) includes the use of novel formulations allowing drugs to be delivered in a controlled manner over a prolonged period of time. Amoxicillin was used as a model drug which is acts on gram +ve and gram –ve organisms. Temperature and pH dependent systems for the treatment of periodontal infections were planned to prepare using poloxamer 407 (18% to 24%w/w) along with carbopol 934 (0.01 to 0.3 % w/w) and Amoxicillin (1%w/w). Poloxamer is a temperature sensitive hydrogel and carbopol 934 is the pH sensitive hydrogel by these combination in-situ gels were prepared and evaluated for physiochemical parameters like gelation Temperature, viscosity, content uniformity, FTIR. In-vitro drug release studies were carried out in Phosphate buffer (6.8 pH) for F1, F5, & F6 formulations .The results of the present study revealed that Gelation temperature for the formulations were F1-35°c , F5-36°c and for F6-38°c which were close to 37°c body temperature. The viscosity of prepared formulations showed that F1 was 29845 (Cps), F5 was 42765 (Cps) and F6 was 45732(cps). The drug uniformity for formulations were between 98.5% to 99.1%, pH of the formulations were F1-6.8, F5-6.9 and F6-6.7, in FTIR showed no interactions between the drug and polymer. The in-vitro diffusion studies showed 79.6% of drug release for formulation F6 when compared to F1 was 95.8% and for F5 was 89.6%. In-situ gels can be easily administered in to the Periodontal Pockets for efficient action of drug dosage forms. Key words: Periodontal infections; Amoxicillin, in-situ gel.