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THE USE OF MICROBIAL TRANSGLUTAMINASES AS A NEW PROMISING DIRECTION IN COMPLEX TREATMENT OF WOUNDS

Yeskova A.Yu.1 , Petrenko А.D.2 , Dydykin S.S.1 , Vasil’ev Y.L 1 , Fomochkina I.I.³ ,Shaymardanova L.R.³, Makalish T.P³, Bessalova Ye.Yu.³

The purpose was to analyse the scientific sources studying the structure and functions of that enzyme in various living species, as well as the possibilities of its use in industry and medicine for wounds healing. Wounds healing are an urgent medical problem, where closure of wounds (ridges convergence) by means of stitches and adhesives is of primary importance, as well as the use of wound healing medicines. Despite the abundance and variety of drugs available on the pharmaceutical market, the local therapy of wound defects of various aetiologies, the desired degree of reparative effect has not been achieved yet. The existing standards of pharmacotherapy of wounds do not satisfy both the effectiveness of treatment and the cost of achieving the final result. In surgery, at the initial stages of the wound process, simple antiseptics and wound-healing ointments are used ("Solcoseryl" and "Metuluracil" are the leaders). The modern level of development of science allowed us to study the wound healing process at the molecular level. In this aspect, the development and implementation of biocompatible materials is an important trend in modern biopharmaceuticals. At present, much attention is paid to the mechanisms of the organization of wound contents and tissue matrix reforming. Transglutaminases (TG enzymes) (serum and tissue) that are capable of forming proteolysis-resistant cross-linking between extracellular matrix proteins are involved in these processes. Drugs of human serum TG in Russia, like all blood products, are produced and used in a limited way. TG of mammals and humans are autoantigens and may be associated with the pathogenesis of certain diseases. At the same time, inexpensive MTGs are widely used in the industry as safe technological agents. TG of microbial origin, unlike oligomeric TGs of mammals and humans, are small single-chain proteins, their activity does not depend on the concentration of calcium ions, they are stable at storage and, at the same time, they are characterized by rapid biodegradation. The idea of creating a new remedy (ointment) containing microbial TG in combination with traditional phytopreparations and active components that affect various targets of the pathological process in tissue damage, and determined the purpose and objectives of our study. At the same time, the mechanisms of reparation should be further investigated in a series of clinical trials. Key words: transglutaminase, wounds healing, pathogenesis, enzyme preparations, collagen synthesis

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