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SCREENING OF ANTIDIABETIC ACTIVITY OF POLYHERBAL COMPOUND ON ALLOXAN INDUCED DIABETIC ANIMAL MODEL

Anitha Nandagopal*, Madiha Jabeen, Anupama Koneru

The ethanolic extract of Nigella sativa, Celastrus paniculatus and Cinnamomum tamala (ENCC) is used in this study to screen the anti diabetic activity in Alloxan induced diabetic rats. Diabetes was induced in rats using Alloxan 150 mg/kg i.p. The study was carried out for 21 days. The phytochemical screening showed the presence of flavonoids, steroids, terpenoids and phemolic compounds. The treatment with ENCC 250 mg/kg and 500 mg/kg effectively increased the body weight of diabetic rats when compared with disease control group. It was discovered that the blood glucose levels and HbA1c levels were reduced in ENCC 250 mg/kg and 500 mg/kg treated groups when compared with disease control group. It also reduced the biochemical parameters like Total cholesterol (TC), Triglycerides (TG), Low density lipoprotein-cholesterol (LDL) and increased the high density lipoprotein-cholesterol (HDL) when compared with disease control group. Histopathlogical studies of pancreas was performed In disease control group, showed apoptosis of beta cells were as treatment with ENCC 250 mg/kg and 500 mg/kg showed mild hypertrophy of islets of pancreas and normal beta cells. Thus, the result suggests that ENCC possesses antibiabetic activity. Keywords: HbA1c, Alloxan, Diabetes, Nigella sativa, Celastrus paniculatus, Cinnamomum tamala, retro orbital plexus.

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