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TITLE:

EVOLUTIONARY RELATIONSHIP OF CODIAEUM VARIEGATUM SPECIES BASED ON SDS-PAGE

AUTHORS:

Abdul Qadir,Nusrat Jahan, Zafar Ahmad, Kamran Taj, Shabir Ahmed

ABSTRACT:

SDS-PAGE is a technique used to separate macromolecules based on their migration through an electric field. Protein may vary in their charge, molecular weight and shape, all of these characteristics will influence their movement in an electric field. The evolutionary relationship based on SDS-PAGE of croton species were observed using SDA-PAGE method. The biochemical and molecular marker enable detailed analysis of genetic relationship, physiological adaptation and variation within population and historical continuity in phylogenetic relationship between various species. The results were recorded based on Nie and lie formula. According to this formula all the varieties were closely related. SDS-PAGE provided valueable information for the identification and evolutionary relationship of croton species. Key word: SDS-PAGE, Codiaem variegatum, Evolutionary relationship.

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