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A SIMPLE METHODS TO DEVELOPMENT AND VALIDATION OF ATORVASTATIN AND BEZAFIBRATE IN THE TABLETS DOSAGE FORMS BY SPECTROPHOTOMETRIC AND SPECTROFLUORIMETRIC.

Nabil A Alhemiary* and Ibrahim A. Alhagri

Developed and validated for the determination of Atorvastatin (ATV) and Bezafibrate (BZF) in the tablets dosage forms using spectrophotometric and Spectrofluorimetric methods. The spectrophotometric methods were based on derivatization of the investigated drugs with two reagents NQS and NBD-Cl. Beer’s law was obeyed over the ranges of 0.3 - 6 and 0.5- 9 µg/mL for NQS reaction, and the ranges of 0.5 - 5 and 1-10 µg/mL for NBD-Cl reaction, the absorbance readings of ATV and BZF, respectively. Spectrofluorimetric methods were developed based on the fact, that the derivative explored drugs with NBD-Cl reagent is very fluorescent items. The fluorescence concentration plots have been linear above the ranges of 0.05–0.5 and 0.5–4 µg /mL for ATV and BZF, respectively. The fluorescence measurement enabled the detection of ATV and BZF at concentrations of about 0.011 and 0.079 µg/mL, respectively. The proposed methods have been validated and efficaciously applied in accordance with the dedication of the investigated drugs of the tablets. The results obtained had been statistically compared to those obtained from reference methods. Keywords: Spectrophotometric, Spectrofluorimetric, Atorvastatin, Bezafibrate, Pharmaceutical analysis.

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