Dr Roshan Abdul Latif, Dr Marrium Anwer, Dr Sumiya bhatti
Objective: To evaluate and compare two different techniques for the detection of H. pylori, ie, endoscopic biopsy and antibodies detection. Study Design: A prospective comparative study based on organization. Place and Duration of Study: In the Medicine Unit II in collaboration with Surgery department of Services Hospital, Lahore for one year duration from Jan 2018 to December 2018. Methodology: The study included 100 patients with dyspepsia who had or were suspected to have H. pylori-associated gastric clinical suspicion or with gastric biopsies and serum samples and clinically suspected patients of both age groups and both sexes. Results: Of the 100 gastric biopsy cases, 90 (90%) had H. pylori. Of these, 52 (57.7%) were male and 38 (42.3%) were female. In ELISA, 68 patients (68%) had antibodies against H. pylori. The male were 38 (55.9%) and females were 30 (44.1%). The specificity and sensitivity of this technique were 75.49% and 60%, respectively. Conclusion: The positivity of H. pylori is very important in histological examination and detection of anti-H. pylori antibodies in Serum using the ELISA method are the simplest and cheapest. Key words: H. pylori, gastric biopsy, giemsa stain.