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TRICHOPHYTON SCHEONLEINII MOLECULAR TYPING BY PCR-RFLP OF THE RIBOSOMAL DNA NON-TRANSCRIBED SPACER REGION

Yasir Ali Butt, Palida Abulizi1,, Xiao Dong-Wang1, Hadiliya-Hasimu

Objective: Tinea capitis is a common children’s disease in the southern part of Xinjiang province, China. Trichophyton schoenleinii is the only causative agent of tinea favosa, which is a relatively common infection in southern Xinjiang. Materials: In the previous study, the molecular epidemiological study of Trichophyton schoenleinii showed that the strains from Xinjiang have different genotypes compared to the strains from mainland China. In this study, we investigated the genetic diversity of 33 T. schoenleinii strains isolated from favus children in Hotan, a famous area in old SilkRoad, which is located at southern Xinjiang. Results: The sequences of rDNA NTS regions of 33 strains were identical. Genotyping study of these strains by PCR-restriction fragment length polymorphism (RFLP) of ribosomal dna (rDNA) non-transcribed spacer (NTS) region used restriction enzyme Ddel showed that 33 strains divided into 10 genotypes. Conclusion: Our result indicated that different geno typicstrains of T. schoenleinii were prevalent among the children in Hotan, and PCR-RFLP analysis of DNA NTS region is a reliable tool for geno typing study of T. schoenleinii. Keywords: Trichophyton scheonleinii; NTS region; PCR-RFLP; Phylogeny; Sequencing.

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