ResearcherID - CLICK HERE Scientific Journal Impact Factor (SJIF-2020) - CLICK HERE

TITLE:

SIMULTANEOUS ESTIMATION OF METFORMIN HYDROCHLORIDE AND GLICLAZIDE IN BULK AND TABLET DOSAGE FORM BY RP-HPLC METHOD

AUTHORS:

Pasupuleti Maneesha Rani1*, Pinnamaneni Prachet, Morla Siva Prasad, Nadendla RamaRao

ABSTRACT:

A simple, precise, rapid, selective, and economic reversed phase high-performance liquid chromatography (RP-HPLC) method has been established for the simultaneous analysis of Lamivudine and Tenofovir Disoproxil Fumarate in Bulk and Pharmaceutical dosage form on a Phenomenex C18 (250×4.6mm i.d) chromatographic column equilibrated with mobile phase containing methanol, acetonitrile & Phosphate buffer (2.954g of potassium dihydrogen orthophosphate in 1000 ml water). Experimental conditions such as pH of mobile phase, organic phase ratio, flow rate, selection of wavelength, etc. were critically studied and the optimum conditions were selected. Efficient chromatographic separation was achieved with mobile phase containing combination of methanol, acetonitrile & phosphate buffer pH 5.0 in ratio of 55:10:35 v/v and mixture was adjusted to pH 5.0 at flow rate of 1.5 ml/min and eluent was monitored at 257 nm. The sample was injected using a 20 μl fixed loop, and the total run time was 5 min. The retention time for Lamivudine and Tenofovir Disoproxil Fumarate were 1.037 min and 2.123 min respectively. The method was linear in the range of 10-50 µg/ml and for both Lamivudine and Tenofovir Disoproxil Fumarate. The proposed method was successfully applied to the analysis of Lamivudine & Tenofovir Disoproxil Fumarate in their Bulk and Pharmaceutical dosage form without interference from other additives. The developed method was validated according to ICH guidelines. Linearity, regression value, recovery, % RSD of method precision, LOD, LOQ values were found within the limits and the method was found to be satisfactory. This validated HPLC procedure is economic, sensitive, user-friendly & less time consuming than other chromatographic procedures. Key words: RP-HPLC, Lamivudine, Tenofovir Disoproxil Fumarate, validation, chromatography.

Top
  • Follows us on
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.