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IMPACT OF SODIUM FLUORIDE ON SPINAL CORD’s GREY MATTER ON ADULT MALE ALBINO RATS

Madiha Amjad, Ayesha Khalid, Memoona Ansari

Objectives: Fluoride is among the elements which is reactive as well as toxic. The exposure of humans to this element is very common from different sources like mouth rinses, normal water for drinking, supplements of diet and toothpastes. The aim of this study is to investigate the impact of NaF alone or in combination with green tea on spinal cords grey matter of albino rats. Methodology: We examined the sections of spinal cord with the utilization of light as well as electron microscopy. Immuno-histochemical evaluations for the manifestation of Caspase-3 and GFAP (Glial Fibrillary Acidic Protein). We used the AGE (Agarose Gel Electrophoresis) for the fragmentation of DNA. Results: The results of this research work showed that sodium fluoride is the cause of production of neuro-degeneration, hemorrhage, vacuolation with abnormal vessels of blood and important rise in the size and amount of the astrocytes. Additionally, neurons displayed an important reduction of their content of Nissl’s granules and significant enhanced Caspase-3 expression. Conclusion: Green tea employed a defensive impact in opposition neurotoxicity induced by sodium fluoride. The findings of this research work add to acknowledged toxic impacts of sodium fluoride. KEYWORDS: Agarose Gel Electrophoresis, Green Tea, Hemorrhage, Astrocytes, Fragmentation, Spinal Cord, Fluoride.