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STRUCTURAL ALTERATIONS IN THE CORNEA OF RAT AFTER MONOCULAR EYELID CLOSURE

Dr Daniyal Sajjad Awan, Dr Serwan Muttayab Mufti, Dr Haseeb Saeed

Objectives: The purpose of this study is to elaborate the histo-morphological alterations in cornea of rat after Monocular Eyelid Closure. Methodology: In this research work, we chose 26 Rattus norvegicus rates of male gender having the age of 3 months. We separated the rats into three different groups; baseline (n: 2), experimental (n: 16) and control group (n: 8). we performed the monocular eyelid closure on these animals chosen for experimentation using ST (Suture Tarsorrhaphy). We euthanized 2 rats from the group of experiment and 2 rats from the group of controls on the experiment days five, ten, fifteen and twenty, we harvested their eyeballs and carried out their routine processing trichrome staining of Masson, paraffin embedding and sectioning. We took out the photo-micrographs with the utilization of the digital photo-microscope. Results: In non-opened eyes, there was a decrease which is time-dependent during corneal epithelium’s stratification with succeeding disintegration and a rise in the dissemination of stromal Keratocyte’s whereas endothelial cells of cornea displayed trivial expansion from the squamous shape. The control eyes and contra-lateral eyes did not show any important alterations in the whole period of experimentation. Conclusion: Monocular Eyelid Closure structural alterations in the epithelium of cornea, stroma and endothelium of suture tarsorrhaphy of eye. Suture Tarsorrhaphy duration cannot be increased because of the danger of the diseases of cornea and reduction of vision acuity as an outcome of the structural alterations. KEYWORDS: Rats, Vision Acuity, Disintegration, Epithelium, Cornea, Experiment, Photo-Microscope.