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TITLE:

THE IDENTIFICATION OF COAGULASE NEGATIVE STAPHYLOCOCCI AS AN EMERGING THREAT AND ITS OPPOSITION WITH REFERENCE TO TIME

AUTHORS:

1Dr. Mirza Ameer Faizan Ali, 2Dr. Yasmin Fatima, 2Dr. Kiran Zahra

ABSTRACT:

Objective: The main purpose of the study is to identify the incidence of seclusion of coagulase-negative staphylococci. Determination of opposition of staphylococci to methicillin with the passage of time is also the objective of the study. Methods: The study was of illustrative and crosswise. It was organized at Services Hospital, Lahore from August 2016 to November 2017. It consisted of patients present in the exhaustive care unit. According to the sample, patients were given suitable culture media through injections. These were kept in inoculation for about one day. Temperature given them during incubation was 35◦C. After that different observation were made on colony like the physical appearance of the colony, staining, positive and negative coagulase tests. Staphylococci were then identified on the basis of these observations. A 30 microgram Cefoxitin disc was used for the identification of methicillin opposing separated staphylococci. Numbers of coagulase opposing staphylococci were calculated every year. Their opposition to the methicillin was also determined. Different samples of staphylococci which were separated during the same time period were compared with each other. Results: Total 1331 samples of patients were studied for about three years. It was observed that from the total of 1331 patients 43.65% were coagulase-negative staphylococci. In 2011 36.5% and in 2012 61% patients with coagulase-negative were identified. It was found that the numbers of patients having negative coagulase were increasing year after year according to the survey. The rates of opposition to methicillin were also found to be enhanced gradually. These were enhanced from 22.7% to 34.3% and again to 59.6% to in 2012. Mostly samples for experiments came from blood about 53.5%. Some samples were also found to come from seepage or scrub which was about 35.1%. Conclusion: It has been concluded that numbers of coagulase-negative staphylococci and its opposition towards methicillin is enhancing observed in the admitted patients in clinics. Keywords: CoNS, Methicillin, MRCoNS.

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