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EFFECT OF PANCREATIC CELLS CULTURE TRANSPLANTATION IN EXPERIMENTAL DIABETES MELLITUS

Mykhaylichenko V. Yu., Zhadko S. I., Samarin S. A., Pilipchuk A. А., Butyrskii A. G., Sulima A. N.

Abstract: Aim. To study possibility of insular and contrainsular hormones in alloxan diabetes after pancreatic cells culture transplantation. Alloxan allows to model diabetes mellitus in rats with similar pathophysiological shifts as in type 1 diabetes mellitus in humans. After transplantation of pancreas cell culture insulin, C-peptide, thyroxine, and triiodothyronine level increases, corticosterone concentration is decreased that is associated with the complete normalization of hormones; this regularity is stable till the end of the experiment. Stabilization of hormones normalizes carbohydrate metabolism in rats. Materials and methods. 80 rats with alloxan diabetes were shared into two equal groups: I – control – non-cured animals, II – experimental – after pancreatic cells culture transplantation. Males and females were even on both groups, animals’ age made 12-14 months, body mass – 244.23±20.31 g. Diabetes mellitus was modeled by subcutaneous introducing Alloxan solution dosed 200 mg/kg. We investigated the main parameters of carbohydrate metabolism (insulin, corticosterone, C-peptide, thyroxin, and triiodothyronine) by radioimmunological method with standard reactives set. Results. In the group after transplantation of pancreatic cellular culture on the 1st day we noticed evident increase of insulin level up to 12.7±0.69 mkIU/l, i.e. 6,6 times more in comparison with database and 3,6 times more above the norm (3,53±0,21 mkIU/l). By the 7th day insulin concentration is decreased down to 3.69±0.07 mkIU/l (p<0,001). By the 14th day insulin level makes 3.45±0.08 mkIU/l that is less of previous index (p<0,05) and does not differ from the norm (p>0.05), and remains within these limits till the end of the 1st month of post-operation period. С-peptide level as well as insulin on the 1st day after transplantation evidently increases 9,8 times comparing with database that is twice more above the norm (0.73±0.02 ng/ml). By the 7th day it unevidently elevates up to 1.59±0.08 ng/ml, and by the 14th day falls for 1,9 times (p<0.001). By the end of the 1st month it remains stable and makes 0.85±0.09 ng/ml that is 5,6 times more above database (p<0.001). Conclusions. Thus, our experiments demonstrated using Alloxane tetrahydrate to model experimental diabetes mellitus and studying different pathological processes in rats. On proved the role of contrainsular hormones misbalance in advanced diabetes mellitus course. Transplantation of pancreatic β-cells normalize hormonal content and glucose level in rats. Key words: alloxan diabetes, cell transplantation, contrainsular hormones.

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