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SIMULTANEOUS ESTIMATION OF KETOROLACTROMETHAMINE AND OFLOXACIN IN OPHTHALMIC PREPARATIONS BY RP-HPLC METHOD

Jahnavi Kutty K, Dr. Elephine Prabhahar A, Ramarao .Nadendla

Abstract: A reversed- phase High Performance Liquid Chromatography (RP-HPLC) method for the rapid and accurate quantification of Ketorolactromethamine and Ofloxacin. The detection was carried out by Shimadzu 250×4.6mm, 5µm column using mixture of buffer KH2PO4 (Potassium dihydrogen phosphate) Ph adjusted to (±)4.0 and Methanol (60:40) with a flow rate of 1.5ml/min. The eluents were detected at 298nm and the Retention time for Ketorolactromethamine and Ofloxacin was found to be 2.395 and 9.779min. Linear responses for Ketorolactromethamine and Ofloxacin were 5-25 µg/ml, 5-25 µg/ml with correlation factor of 0.998 and 0.998 respectively. The method was validated as per ICH guidelines for specificity, linearity, accuracy and robustness. The validated method was successfully employed for the simultaneous determination Ketorolactromethamine and Ofloxacin. Key Words: Ketorolactromethamine , Ofloxacin, RP-HPLC, NSAID.

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