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ANALYSIS OF F HUMAN EPIDERMAL GROWTH FACTOR RECEPTOR 2 (HER2/NEU) EXPRESSION IN GASTRIC ADENOCARCINOMA

Dr Nadia Umar, Dr Waqar Shad, Dr Maria Irfan

Introduction: Gastric carcinoma is a substantial health-care problem worldwide, with over 6.8% of total new cancer diagnoses and 8.8% of total cancer-related deaths annually. Aims and objectives: The main objective of the study is to analyse the human epidermal growth factor receptor 2 (Her2/Neu) expression in gastric adenocarcinoma. Material and methods: This descriptive study was conducted in Ayub Medical College, Abottabad during October 2018 to March 2019. The data was collected from 100 gastric adenocarcinoma patients. Formalin-fixed, paraffin-embedded samples of tumors and corresponding normal stomach tissues from gastric cancer patients were evaluated for HER2 protein and gene amplification using immunohistochemistry (IHC) and fluorescence in-situ hybridization (FISH) analysis. Results: The data was collected from 100 patients of adenocarcinoma. The median age was 62 years. Of all the tumors examined, 31 (15.74%) were located in the cardiac region, 42 (21.32%) in the body, and 122 (61.93%) in the pylorus. The majority (98.98%) of the samples were primary tumors with only 2 recurrent tumors identified. According to Lauren classification, 63 (31.98%) tumors were intestinal-type and 134 (68.02%) were diffuse-type or mixed-type carcinomas. Conclusion: It is concluded that HER-2/NEU is significantly overexpressed in low-grade gastric carcinoma, and overexpression is not dependent on various other clinicopathological parameters.

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