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TITLE:

ANALYSIS OF HUMAN PAPILLOMAVIRUS IN ARCHIVED CLINICAL SAMPLES OF BREAST CANCER PATIENTS

AUTHORS:

Dr Shehroz Rana, Dr Shahid Iqbal, Dr Sundus Bukhari

ABSTRACT:

Introduction: Breast cancer is the most common cancer in women worldwide, and there were representing 22.9% of all new cancers in 2008 (an estimated 1.378 million new cases) and ranking second overall when both sexes are considered together. Aims and objectives: The basic aim of the study is to analyse the human papillomavirus in archived clinical samples of breast cancer patients. Material and methods: This descriptive study was conducted in health department of Punjab during October 2018 to March 2019. The data was collected from 50 patients of breast cancer. This study aimed to analyse the human papillomavirus in breast cancer patients. Genomic DNAs from tissue sections were prepared. For genotyping of HPV, the positive PCR products were analyzed by sequencing. The DNA sequence was determined with the Big-Dye terminator cycle sequence kit and an ABI 377A sequencer. Results: The data was collected from 50 patients. Statistical difference was observed in the HPV DNA status and HPV genotype between these two groups. HPV infection has increased the risk of breast cancer and had an OR of 13.953. The high-risk HPV types (HPV 16 and 18) were more prevalent than other HPV types in the cases. The HPV genotypes in samples of breast cancer patients were 26.67% for HPV-16 (4 isolates) and HPV-18 (4 isolates), 13.3% for HPV-23 (2 isolates) and HPV-6 (2 isolates), 6.67% for HPV-11 (1 isolate), HPV-15 (1 isolate), and HPV-124 (1 isolate), and one isolate could not be genotyped compared to HPV reference sequences while the sole detected HPV in control specimens was HPV-124. Conclusion: It is concluded that the presence of HPV genome in tumor tissues in women with breast cancer. HPV infection is associated with the development of breast cancer in women.

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