Dr Shehzina Saeed, Dr Ayesha Nawaz, Dr Fizza Khalid
Introduction: Surgical site infections (SSIs) consists of both infected bones and infected tissues and are the microbial contamination of surgical or operative wounds that occur within 30 days of a surgery or within 1 year post-surgery. Infections associated with surgical incisions are called SSIs. Objectives: The main objective of the study is to find the pathogenic bacteria isolated from tissues, bones infections and their antibiotic susceptibility pattern. Material and methods: This descriptive study was conducted in health department Punjab during July 2019 to December 2019. The data was collected from 100 patients having SSI, Infected bones and tissues which associated with Austin Moore prosthesis, tibial plating and humerus plating. The collected SSIs samples were inoculated on different Media like; Blood agar, MacConkey agar and Mannitol Salt Agar (MSA) media. Growth was observed after 24-48 hours incubation at 37°C aerobically. Results: The data was collected from 100 patients having SSI. On culture of these specimens, 28 showed no growth, while 72 (87.17%) specimens were found to have microbial pathogens. A total of 437 microbial organisms (412 bacteria and 25 Candida species) were isolated in 272 specimens. Escherichia coli was the most frequent species, which isolated from tissue specimens (46:46.46%) specimens, followed by Staphylococcus aureus in (42:42.42%), and Pseudomonas aeruginosa was found in (37:37.37%). Conclusion: It is concluded that a high rate of infection in our study as compare to the previous studies in this regard which means that sophisticated preventive measures are not implemented.