Praveen Sivadasu*, Boppudi Bindu Sri, Gorre Asha, Bhukya Chandana, Abdul Safiya Naseema, Mamillapalli Sneha Latha, Padmalatha K
Background and Aim: Periodontitis is one of the widest spread oral diseases. Medicated in-situ gels of Ciprofloxacin Hydrochloride (CH) for an extended period of retention in the infected cavity were prepared for improved local action for the treatment of periodontitis. Methods: Medicated formulations were prepared using ion-sensitive (badam gum) and pH-sensitive (carbopol 934P) polymers. The central composite design was employed and prepared batches were characterized by FTIR, pH, syringeability, drug content, clarity, gelation temperature, gelling time, in-vitro gelling capacity, in-vitro diffusion study. Results: Gelation temperature, (in-vitro) gelling time and the nature of gel formed in simulated saliva showed polymeric concentration dependency. The diffusion study of optimized in-situ gel had been performed which showed augmented arrival of medication from 7-10 hours and the discharge was dependent on polymer utilized. In vitro anti-microbial study was carried out by utilizing E.coli and S.aureus and the results suggested that formulated in situ gel have better anti-microbial activity when compared with pure CH. Conclusion: The formulated CH loaded in situ gel can be used as an alternative approach to treat periodontitis. Keywords: Periodontitis; In situ gel; Sustained drug delivery; Ciprofloxacin hydrochloride; Polymers; Central composite design.