::INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES::

CODEN : IAJPBB
issn 2349-7750

News Updates Latest Volumes: COSMOS IMPACT FACTOR (2018)- 4.153, GLOBAL IMPACT FACTOR(2014) - 0.384, (2015) - 0.437, Indo American Journal of Pharmaceutical Sciences,

ResearcherID - CLICK HERE Scientific Journal Impact Factor (SJIF-2020) - CLICK HERE

TITLE:

BACTERIAL PATHOGENS RESPONSIBLE FOR BLOOD STREAM INFECTIONS AND THEIR DRUG SUSCEPTIBILITY IN A TERTIARY CARE HOSPITAL

AUTHORS:

Dr Muhammad Bilal, Dr Ali Raza, Dr Hummayoon Ashraf

ABSTRACT:

Objective: To determine the pattern of bacterial agents responsible for blood stream infections (BSI) Methods: Cross sectional descriptive study was carried out in the Pathology departments of Benazir Bhutto Hospital and Holy Family Hospital Rawalpindi during the period October, 2015 to May, 2017. Blood culture of 1284 bottles containing appropriate amount of blood received from indoor and outdoor patients. Blood agar and MacConkey agar plates were used to subculture the collected sample. The sensitivity of the causative organisms was obtained by collecting samples using antiseptic measures and culturing on BHF culture medium. Results: Gram Negative bacterial growth is responsible for blood infection in 93.3% of cases whereas 6.7% of bloodstream infections are caused by Gram Positive bacterial growth. Mono microbial agent growth causes 92.6% blood stream infections and poly microbial agents cause 7.4% blood stream infection. Bloodstream infections caused by Gram Positive Bacteria are associated with S. Aureus is 38.8% and 1.9% are associated with Streptococcus spp. In bloodstream infections caused by Gram Negative bacteria, Salmonella spp is responsible for 14.2% cases, Klebsiella spp is responsible for 13.5% cases, Acinetobacter spp is responsible for 11.6% cases, E.coli and Pseudomonas are responsible for 6.5% of cases, enterococcus spp is responsible for 3.2 % of cases, proteus and Enterobacter spp is responsible for 6% of cases, candida spp is responsible for 2.5 % of cases. salmonella specie showed susceptibility (100%) to Aztreonam, ceftriaxone, Moxifloxacin, Tigecyclin and meropenam. Klebsiella specie showed susceptibility (100%) to Cefaclor, tegeyclin and vancomycin. Pseudomonas shows susceptibility (100%) to moxifloxacin and cotrimoxazole. Enterococcus shows susceptibility (100%) to linezolid and vancomycin. E.coli shows susceptibility (100%) to gentamycin and meropenam. Streptococcus shows susceptibility (100%) to Augmentin, Ciprofloxacin, Doxycycline, Fusidic Acid, Moxifloxacin, Vancomycin and Staphylococcus Aureus shows susceptibility (100%) to Tigecyclin, Vancomycin. Conclusion: Gram negative bacteria involving mono microbial agent growth is responsible for most of blood stream infections Keywords: Blood stream infections, Benazir Bhutto Hospital, Holy family Hospital, antibiotic Sensitivity Pattern.