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BIOTECHNOLOGY CULTIVATING THE PHYSIOLOGICALLY ADAPTED LACTOBACILLI TO CREATE MICROBIAL BIO-ADDITIVE FOR POULTRY

Andrey Koshchaev, Oksana Takhumova, Ruslan Omarov, Sergei Shlykov, Nina Konik

This article presents the results of research on the selection of the most suitable nutrient medium, ensuring maximum biomass growth of lactic acid microorganisms. The objects of research were pure cultures of microflora of the gastrointestinal tract of quails - Lactobacillus agilis, Lactobacillus intermedius and Lactobacillus salivarius. For the cultivation of microorganisms used 4 nutrient medium: medium for lactic acid microorganisms (Uglich), molasses autolysate medium (MAM), glucose-peptone medium (GPM) and medium MRS. In the cultivation of bacteria, the time and temperature of growth served as variable parameters. In the process of cultivation, the analysis of the dynamics of consumption of reducing substances and the titer of microorganisms was carried out. According to the results of the cultivation of microbial cultures, an active consumption of the carbon substrate was found in the nutrient media variants used, it was found that by 24 h of cultivation a maximum of cells was observed. Based on the experiments performed, it was established that with different parameters, the highest cell titer was achieved by 24 h at a temperature of 38.0 ° C on molasses autolysate medium. Thus, molasses autolysate medium can be recommended under production conditions as the cheapest substrate for the further development of probiotic biologics for the poultry industry. Key words: lactobacilli, nutrient medium, cultivation, titer, dynamics, temperature, time, reducing substances, microscopy, morphology.

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