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TITLE:

USE OF BLOOD PLASMA AS AN EXTRACTANT OF BIOLOGICALLY ACTIVE SUBSTANCES FROM PLANT DIGESTION

AUTHORS:

Ruslan Omarov, Sergei Shlykov, Anton Nesterenko, Dmitry Ryakhovsky

ABSTRACT:

Development of functional beverages with extracts from raw materials of plant origin, containing many biologically active components remains the most promising direction in the creation of healthy foods. Special interest is production the protein-containing drinks, while along with traditional milk drinks products based on animal blood plasma deserves special attention. This paper contains the results of studying of possibility to use hydrolyzed blood plasma as an extractant in preparation a base for producing functional beverages. The investigations were carried out into laboratories of the department of technology of production and processing of agricultural products of the Stavropol State Agrarian University. As objects of research were used: peppermint, carcade, lentils, chickpea, hydrolyzed blood plasma and extracts obtained on its basis. Hydrolyzed blood plasma was obtained by pretreatment with collagenase enzyme: protein concentration was 4.0–4.5%, enzyme concentration was 0.37% (with an activity of 900 uPA/g), the hydrolysis temperature - 37-42 °C, the pH - 6.6-7.1, hydrolysis duration 2.5 hours, hydrolysis degree of plasma proteins was about 82%. Data were analyzed for significance using Statistical Analysis System software version 9.1. By calculation was established that the plant extracts have a high biological value, which confirms the good balance of the amino acid composition. Studying of storage ability revealed the absence of pathogenic microflora into the dry product with a shelf life of up to 12 weeks by temperature not higher than 4 °C. The indicator KMAFAnM remained within 2.4 × 103 CFU per 1 g of the product. Thus, using the hydrolyzed blood plasma as an extractant of plant raw materials can be recommended as the basis for producing functional beverages with high biological value. Keywords:blood plasma, functional drinks, plant extracts, extraction.

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