*Dr Satyjeet, *Dr. Sumeet Kumar, *Dr. Akash Mandhan, *Dr. Aroon Kumar
Diabetic nephropathy in insulin dependent patients is believed to be characterized by glucose-induced inhibition of matrix-disrupting enzymes to contribute to matrix deposition. Objective: The study aimed to explore renal tubular proteins that can detect an early stage of renal involvement in diabetes. Study design: A Prospective Study. Place and Duration: In the Nephrology department of Chandka Medical College Hospital Larkana for one year duration from July 2017 to July 2018. Methods: The study included 42 female patients aged between 40 and 60 years with long-term diabetes and 27 male patients with insulin (18-20 units). Electrophoretic patterns of 24-hour urine proteins in diabetic patients were examined in 10% SDS gradient polyacrylamide gel electrophoresis. We estimated 24 hours urine protein from patients. Results: The electrophoretic profile showed a proteinuria status (both high molecular weight and low molecular weight). In the urine samples, proteins with a molecular weight of 120-28 K have appeared. In normal subjects, the urinary protein range is 37-72 Kda. Among these, common proteins and control urine samples observed in patients were protein 27 molecular weight (alpha 1 microglobulin) and 42 Kda (acid glycoprotein). Gross volume (concentration) and density of these 2 proteins were observed in the urine samples of the patients. Conclusion: Electrophoresis technique may exclude or detect tubular-interstitial nephropathies in early stages of renal complications, as in diabetes mellitus. Key words: Type 11 diabetes, alpha-1-acid glycoprotein, SDS electrophoresis.