::INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES::

CODEN : IAJPBB
issn 2349-7750

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TITLE:

METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF ISONIAZID IN TABLET DOSAGE FORM BY UV VISIBLE SPECTROSCOPY

AUTHORS:

Venkat Reddy. R, J.Bhagyarani, Rahmath Fathima, S.Likitha, Santhosh Illendula, K.N.V. Rao, K. Rajeswar Dutt.

ABSTRACT:

Tuberculosis (TB) is the world’s second most common cause of death from infectious disease, next to human immunodeficiency virus (HIV). TB has become an increasing health problem since emergence of HIV; TB/HIV co-infection adds further complications in treating the disease. It is also a major concern for industrialized nations because of emergence of drug resistance, alcohol / drug abuse, growth of immigrants and other factors. Method employs formation and solving of simultaneous equation using 263 nm and 292 nm as two analytical wavelengths for both drugs in Distilled water. Isoniazid and at their respective λmax 263 nm and 292 nm shows linearity in a concentration range of 1-11μg /mL and 5-30μg /ml. Recovery studies for Isoniazid 98% and 102% for in case of simultaneous equation method confirming the accuracy of the proposed method. The proposed method is recommended for routine analysis since it is rapid, simple, accurate and also sensitive and specific. Keywords: UV - Visible Spectroscopy, Isoniazid, Distilled water.