ResearcherID - CLICK HERE Scientific Journal Impact Factor (SJIF-2020) - CLICK HERE

ANALYSIS OF FRAGMENTED DNA IN INFERTILE MALE EJACULATE IN DUKAGJINI PLAIN IN THE REPUBLIC OF KOSOVO

Afrim Zeqiraj, Agim Shabani, Zehra Hajrulai-Musliu, Sadi Bexheti, Nexhbedin Beadini, Sheqibe Beadini, Hesat Aliu, Lutfi Zylbeari, Nasir Bexheti, Zafer Gashi, Shkelzen Elezaj

Aim: Infertility is defined as without the ability of a sexually active mate to reach a pregnancy despite regular and unprotected sexual intercourse for more than 12 months. Analysis of fragmented DNA in the sperm. The fragmented DNA analysis was performed according to the procedure mentioned in the instruction of the Halosperm / Halotech DNA reagent, S.L. Madrid, Spain. Materials and methods: The statistical processing of the data is done with the statistical package SPSS 22.0. Patients received for analysis were all from the Republic of Kosovo. The sampling period was 2014/2018. All analyses were performed at Biolab Zafi, Laboratory in Peja, Republic of Kosovo. The difference is significant if P <0.05. Results: The study included 257 males of different age groups, of whom 169 were in the study group and 88 in the control group. In the study group were men with asthenospermia, oligospermia and oligoasthenospermia, whereas in the control group were men with normospermia. Conclusions: We can conclude that the results of DNA fragmentation fragmented into the sperm are encouraging and can be used for diagnostic purposes in determining male infertility. Such an assessment of DNA damage to the sperm can serve clinicians to assist spousal couples with infertility in the selection of IVF / ICSI assisted medical support technique. It is recommended that such an analysis of DNA fragmentation determination in the sperm becomes part of the clinical trials of Andrology laboratories. Key words: Sperm DNA fragmentation, Kit Halotech/Halosperm, IVF / ICSI.

FULL TEXT