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ANALYSIS OF EXPRESSION OF P-AMPK IN COLORECTAL CANCER SURVIVAL PATTERNS

Dr Jamal Yousaf, Dr Ayesha Aziz, Dr Afrasyab Chatha

Abstract: Introduction: Colorectal cancer (CRC) is the third most common tumour worldwide and represents the second leading cause of cancer death in Europe and the USA. Aims and objectives: The main objective of the study is to analyze the expression of P-AMPK in colorectal cancer survival patterns. Material and methods: This descriptive study was conducted in CMH, Rawalpindi during April 2018 to October 2018. This study was done for the analysis of expression of P-AMPK in colorectal cancer patients. The data was collected from 50 cancer patients. Expression of P-AMPK was measured with immunoblotting. For a complete list of the cell lines, antibodies, and reagents, see the supplemental material. Proteins were resolved using SDS-PAGE and transferred to nitrocellulose membranes, blocked in Odyssey blocking buffer (Li-Cor), hybridized with primary and secondary antibodies in Tris-buffered saline (TBS)–0.1% Tween 20, and detected using an Odyssey imaging system. Significance of the data was calculated using SPSS version 19.0. Results: The data was collected from 50 colorectal cancer patients. Phosphorylated AMPK expression was associated with p-MAPK3/1 expression (P<0.0001) and inversely with high tumour grade (P=0.0009), MSI-high (P=0.0021) and CIMP-high (P<0.0001). In 16 of 20 sections, tumour centre and tumour invasive front showed concordant expression status, indicating that p-AMPK expressions in tumour centre and invasive front were not different in most cases. Conclusion: It is concluded that AMPK activation is associated with good prognosis among MAPK3/1-activated colorectal cancer patients, while AMPK activation is not associated with prognosis among MAPK3/1-inactive cancer patients.

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