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ANALYSIS OF LEVEL OF MUSCLE GLYCOGEN CONTENT IN TYPE 2 DIABETES IN PAKISTAN

Dr Azizullah, Dr Sohail Abbas Juwa, Dr Jibran ul Haq

Abstract: Introduction: Glycogen metabolism is controlled predominantly by the coordinated action of two enzymes, glycogen synthase and glycogen phosphorylase, both of which are regulated by phosphorylation and allosteric modulators. Aims and objectives: The basic aim of the study is to analyze the level of muscle glycogen content in type 2 diabetes in Pakistan. Methodology of the study: This cross sectional study was conducted in Holy Family Hospital Rawalpindi during May 2018 till November 2018. The data was collected from 100 diabetic patients who visited the OPD of the hospital. 5cc blood sample was collected from each patients.Blood was centrifuged and separated the serum for further analysis. Serum creatine phosphorkinase (CPK) levels were measured to exclude skeletal muscle disorder. The EDL muscle of all patients was assessed for glycogen content, using an anthrone based method. Serum free carnitine levels were measured from blood serum using L-carnitine assay kit. Results: The data was collected from 100 diabetic patients. The mean age of the selected patients was 55.66±4.56 years, fasting serum TG (101.38 ± 7.15mg/dl), fasting serum HDL (79.65 ± 5.11mg/dl) in all selected patients. The serum free carnitine was measured by terminal sampling and glycogen content of the EDL muscle were calculated. An increase in glycogen content was observed in diabetic group (82.55±10.30 mg/100 gm muscle). The serum free carnitine levels showed a significant rise in diabetic group.

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