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A COMPREHENSIVE STUDY ON INTERACTION OF ANTIMICROBIAL PEPTIDES AS A TREATMENT OF TUBERCULOSIS

Dr Iram Hassan, Dr Ujala, Dr Aneeza Ilyas

Abstract: Introduction: Mycobacterium tuberculosis (Mtb) are classified as acid-fast Gram-positive bacteria due to their lack of a typical outer cell membrane. Contagious tuberculosis spreads through the air. Objectives of the study: The main objective of the study is to analyze the interaction of antimicrobial peptides as a treatment of tuberculosis. Methodology of the study: This cross-sectional study was conducted at Services Hospital Lahore during February 2018 till July 2018. The data was collected from 100 patients of both genders. Those patients who suffered from TB and visited the OPD of the hospital included in this study. In this analyzes we compare the different drugs of TB which are available in the market as compared to antimicrobial peptides. A reasonable approach to find new therapeutics against the high number of MDR and XDR strains is to find molecules having a different killing mechanism. Results: The data was collected from 100 TB patients. The mean age of the selected patients was 50.98±4.56 years. Peptide concentrations of 1, 10 and 100 μg/ml, 10-fold serial concentrations were used. After 7 days incubation with peptides, the CFU/ml of each sample was calculated compared with day 0. The samples treated with D1 or D5 at 100 μg/ml had dramatic reductions in CFU/ml. eptide D5 increased the anti-tuberculosis activity by about 5.7-fold compared to D4, which is due to a valine to lysine substitution at position 16. Conclusion: It is concluded that Many AMPs have good or moderate activities against mycobacteria, however, in general the activities are lower as compared to the activities found against other Gram-negative or Gram-positive bacteria.

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