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ANALYSIS OF SALIVARY BIOMARKERS INTERLEUKIN-8 LEVELS CORRELATED WITH DIFFERENT PARAMETERS OF NASWAR

Dr Ameena Najam, Dr Humaira Akhtar, Dr Muhammad Farhan Raza

Abstract: Introduction: Almost one-fifth of the tobacco used in the world is smokeless form. Smokeless tobacco products are highly addictive due to their high nicotine content. Aims and objectives: The basic purpose of this study was to evaluate the salivary biomarkers interleukin-8 levels correlated with different parameters of naswar. Material and methods: This cross sectional study was conducted in August 2018 to January 2019 at Nishter Dental College, Multan. The data was collected from 100 patients who were used naswar from last one year. The data was collected through a non-probability sampling technique. The age range for this study was 30 to 60 years. Age, duration of use of naswar, frequency of use, type of naswar used and site of placement of naswar in oral cavity were noted. A morning sample of unstimulated whole saliva was collected. ollected samples were tested for levels of interleukin-8 cytokine by enzyme-linked immunosorbent assay (ELISA) procedure. Frequancy of usage of naswar were also noted. Results: The data was collected from 100 participants. The mean age of the participants was 35.65 years. Levels of salivary IL-8 were found from 173. 48 pg/ml to 296.78 pg/ml with mean and standard deviation of 173.48±46.52pg/ml. Naswar dippers mostly belonged to the Pathan community (76.2%). Regarding educational status in naswar users and non-users, 59.5% and 36.4% were uneducated, respectively. Most common reason for using naswar was that they were just addicted to it. Conclusion: It is concluded that there was a high level of IL-8 were present in naswar users. There is a positive correlation between salivary biomarkers and IL-8 levels and frequency of naswar usage.

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