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"ISOLATION AND CHARACTERIZATION OF L-ASPARAGINASE-PRODUCING ACTINOMYCETES FROM MANGROVE SEDIMENTS: INSIGHTS INTO ANTI-MICROBIAL AND ENZYMATIC POTENTIAL"

G. Madhavi and Vidyadhara S

In recent decades, microorganisms have been shown to store bioactive chemicals. Actinobacteria manufacture secondary metabolites most efficiently. The rapid spread of epidemic illnesses, drug-resistant microorganisms, and the need for effective anti-cancer drugs need the development of effective antibiotics. Several actinobacterial species have been studied for bioactive chemical production from terrestrial environments in recent decades. Sediment samples from Nizampatnam (Station I) and Coringa (Station II) mangrove habitats were analysed using standard procedures. Parameters recorded included moisture content (15%), 17% (Station II), pH (7.6 and 7.2), organic carbon (7.2 and 6.8), and total nitrogen (4.54 and 4.92 µg/g). Air-dried sediment samples were pre-treated with four methods and plated on three selective medium employing soil dilution to extract and count actinomycete colonies (Yi., 2019). Calcium carbonate pre-treatments had higher actinobacterial counts than dry heat, phenol, and ringer's solution. Compared to asparagine glucose agar (AGA) (Smith, 1943) and glycerol-asparagine agar (GAA) (Waksman, 1961), starch-casein agar media (Wellington and Cross, 1961) promotes actinomycete growth. Nizampatnam mangroves yielded more actinobacterial strains than Coringa. Purified actinomycete strains were cultivated on yeast extract-malt extract-dextrose (ISP-2) agar. Keywords: Enzymatic Potential, L-Asparaginase, Mangrove Sediments.

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