::INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES::

CODEN : IAJPBB
issn 2349-7750

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TITLE:

THE ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF POVIDONE & ORINIDAZOLE BY USING RP- HPLC

AUTHORS:

K.Aruna kumar*, Santanu Kumar Hotta,Chaitanya Bangari , Dr.M.B.VenkatapatiRaju

ABSTRACT:

A new method was established for simultaneous estimation of Povidone and Ornidazole by RP-HPLC method. The chromatographic conditions were successfully developed for the separation of Povidone and Ornidazole by using Inertsil C18 column (4.6×150mm)5µ, flow rate was 1ml/min, mobile phase ratio was (70:30 v/v) methanol: Phosphate buffer, detection wavelength was 240 nm. The instrument used was WATERS HPLC Auto Sampler, Separation module 2695, photo diode array detector 996, Empower-software version-2. The retention times were found to be 2.462 mins and 3.737 mins. The % purity of Povidone and Ornidazole was found to be 99.84% and 100.27% respectively. The system suitability parameters for Povidone and Ornidazole such as theoretical plates and tailing factor were found to be 5358.3, 1.2 and 7597 and 1.1, the resolution was found to be 8.4. The analytical method was validated according to ICH guidelines (ICH, Q2 (R1)). The linearity study of Povidone and Ornidazole was found in concentration range of 10µg-50µg and 25µg-125µg and correlation coefficient (r2) was found to be 0.999 and 0.999, % recovery was found to be 99.96% and 99.98%, %RSD for repeatability was 0.3 and 0.7, % RSD for intermediate precision was 0.3 and 0.9 respectively. The precision study was precision, robustness and repeatabilty.LOD value was 3.17 and 0.2372 and LOQ value was 7.80 and 5.30 respectively. Keywords: Povidone , Ornidazole ,precision, robustness and repeatability.