::INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES::

CODEN : IAJPBB
issn 2349-7750

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TITLE:

THE ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF OFLOXACIN & TINIDAZOLE BY USING RP- HPLC

AUTHORS:

P. Valli Sowjanya*, Santanu Kumar Hotta, Chaitanya Bangari, Dr.M.B.Venkatapathi Raju

ABSTRACT:

The present work deals with ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF OFLOXACIN AND TINIDAZOLE IN TABLETS BY RP-HPLC using INERTSIL ODS 3V column,C18(250x4.6 ID). A mobile phase consisting of phosphate buffer: Acetonitrile buffer , pH 3.5 adjusted using Orthophosphoricacid in 50:50v/v ratio was employed in this study. The flow rate was set at 1.2 ml/min. Separation was performed at ambient temperature. Eluents were monitored by UV detector set at 308nm. The developed method was statistically validated for linearity and range, precision, limit of detection, limit of quantification, accuracy, robustness, Ruggedness and specificity. Keywords: phosphate buffer ,Acetonitrile, linearity , range, precision, limit of detection, limit of quantification, accuracy, robustness, Ruggedness and specificity.