N. Puranik*, A. Saxena, A. Kapoor
Ethanolic extract of Ocimum tenuiflorum leaves was prepared and subjected for preliminary phytochemical screening, antioxidant activity using 1,1 Diphenyl 2 picryl hydrazyl radical scavenging assay, protective roles at various levels as ex vivo lipid peroxidation, Anhti haemolytic assay and inhibition of protein carbonyl. Following ehtanolic extraction O. tenuiflorum exhibit some important phyotchemicals as flavanoids, tannins, terpenoids and coumanrins, which are important from the medicinal and pharmacological point of view. Moreover in O. tenuiflorum reflected potent antioxidant and protective capacity to quench DPPH radical, inhibiting lipid peroxidation, Inhibiting erythrocyte heamolysis and prevention of phenyl hydrazone generation in protein carbonylation in a dose dependent manner even at very small concentrations ranging 1, 5, 10, 25 and 50 µg/ml. All the test results evedent potent antioxidant and protective capacity of O. tenuiflorum at in vitro level. Key words: Ocimum tenuiflorum, herbs, holy basil, Antioxidant, Lipid peroxidation, protein carbonyl, protective role.