Dr. Madeeha Afaq, Dr. Misbah Batool, Dr. Irum Balooch
Objectives: The main objective of this study was to determine the levels of antioxidants after the use of Ropivacaine for anesthesia. Material and method: This cross-sectional study was conducted in Nishtar Hospital, Multan during March 2019 to December 2020. To measure the levels of lipid peroxidation, glutathione (GSH), superoxide dismutase (SOD) and the levels of catalases (CAT), samples were taken from the vein before anesthesia (0 min) and at 5, 15, and 60 min. after anesthesia. Results: The levels of MDA , SOD and GSH slightly increased at 5 min after spinal injection of ropivacaine and this increase continued throughout anesthesia (P<0.001). Catalases levels were decreased at 5 min after anesthesia (P<0.001) and this level not recovered after 60 min of anaesthesia. Conclusion: In conclusion, we found that spinal injection of ropivacaine increased free radical levels in spinal ﬂuid which may be supported antioxidant environment of spinal ﬂuid during anesthesia.