::INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES::

CODEN : IAJPBB
issn 2349-7750

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TITLE:

ANALYSIS OF BACTEC TEST OF CSF IN CNS TUBERCULOSIS

AUTHORS:

Dr. Faisal Atta, Dr. Armghan Khalid, Dr. Ussama Iftikhar

ABSTRACT:

Introduction: In Pakistan, tuberculosis is generalized and wide spread. There have been two prevalence surveys conducted in 1960-62 and 1974-78 with similar results. According to these surveys 54% of the entire population is infected and this infection rate goes as high as 80% in age groups of 20-29 years and above. Objectives: The main objective of the study is to analyze Bactec test of CSF in CNS Tuberculosis. Methodology of the study: This descriptive study was conducted at Services Hospital Lahore during June 2019 to February 2020. We follow the following procedure for the analysis of TB in local population of Pakistan. The collected blood vials were tested every 2nd day for the first 2 weeks and weekly thereafter for a total of 6 weeks. The Bactec TB medium (12B) is an enriched middle-brook 7H9 broth base. Mycobacteria utilize a 14C labelled substrate (fatty acid) present in the medium and release 14CO2 into the atmosphere above the medium. Results: A total 100 patients were included in the study. Out of these 100 patients 30 (30.94%) patients were male and 70 (70.05%) were females with male to female ratio of 1.03:1. This study was conducted in age groups 2 month to 14 years. Maximum number of cases 32(60.37%) were between 1-5 years. Conclusion: It is concluded that Polymerase Chain Reaction is the method for Rapid diagnosis of Tuberculous Meningitis. PCR was done on CSF and it was positive in 50.94% compared to Bactec and conventional smear and culture which showed positivity of only 15.09% & 3.77% respectively.