Objective: This study is aimed to observe the characteristics of the morphometric analysis in mutirooted teeth. Materials and Methods: In this study 104 mandibular and maxillary molars were included in which 33 were first molars of mandibular and 19 were the second molar of mandibular whereas the first molars of maxillary were 34 and second molars of maxillary were 18. With the help of micro caliber interradicular width space, vertical dimensions and roots length was measured. In mandibular and maxillary various type of root trunk were also observed. Using t-test statistical analysis was also done Results: From 1st to 2nd molar the length of roots was considerably reduced. In mandibular molars this decrease in length was more prominent. In mandibular molars the prevalence of root trunk B was 51.9% and in maxillary molars this was 67.3%. In both molars the length of root trunk was observed to be increased from 1st to 2nd molar. Interradicular width space between and on same teeth also varied. Conclusion: This study provides epidemiological data about root trunk vertical dimension, interradicular space and molars root length in a Tunisian population that could help clinicians in endodontic and periodontal therapy.