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PHYLOGENETIC BIODIVERSITY OF YEASTS IN THE REPUBLIC OF NORTH OSSETIA-ALANIA (first report)

*Boris Georgievich Tsugkiev, Alan Makarovich Khoziev, David Nikolayevich Khanikaev, Valentina Batyrbekovna Tsugkieva, Andrey Georgievich Petrukovich, Eteri Illarionovna Rekhviashvili, Ruslan Gelbertovich Kabisov

The authors identified yeast strains from the surface of grape cultivars Interes (strain 3K (14) and Dekabrskiy (strain 5e), as well as from the wild hops cones (strain BUG1) and performed their identification. The primary identification of the selected strains was performed by studying cultural, tinctorial, morphological, physiological-biochemical, and technological properties in the Research Institute of Biotechnology of Gorsky SAU, using traditional research methods. For the final identification based on the analysis of ribosomal genes sequence, 3K (14), 5e, BUG1 strains were sent to the Bioresource Centre – All-Russian Collection of Industrial Microorganisms (BRC VKPM) National Research Centre “Kurchatov Institute” – State Research Institute of Genetics. According to the results of the performed in BRC VKPM NRC “Kurchatov Institute” – State Research Institute of Genetics analysis of the nucleotide sequence, coding part of genes rRNA it was found that 3K (14) strain is the closest to the species Metschnikowia pulcherrima; BUG1 strain is closest to the species Saccharomyces; 5e strain is closest to the species Rhodotorula mucilaginosa. Keywords: yeasts, strain, gene encoding 18S rRNA, DNA, PCR mode.

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