Dr. Asma Aslam, Dr. Manseba Zahid, Dr. Murtaza Khan
Aim: To investigate the frequency and clinical correlation of hepatitis B for common genotypes with PCR. Place and Duration: In the Gastroenterology department of District Headquarter Hospital Sargodha for one year duration from February 2019 to February 2020. Methods: The HBV genotype was determined on the basis of 206 serum samples from patients with chronic HBV infection. DNA was extracted from HBsAg and HbeAg positive cases, and their genotypes were determined using species-specific primers. Results: All genotypes were specified in patients except genotype E. Genotypes B, C and A, were the predominant ones, appearing in 48 samples (23.3%), 57 samples (27.6%) and 43(20.8%), while genotypes, D and F were appearing in 21(10.1%) and 4(2%) of samples, respectively. However, 33(16.2%) samples were determined as mixed genotype. At the beginning of the study, patients infected with HBV genotype C were generally younger with female dominance at the border. HbeAg to anti-Hbe seroconversion occurs much earlier in genotype B than in carriers of genotype C. Higher levels of HBV DNA were detected in patients infected with genotype C compared to four other genotypes. Conclusion: HBV genotype plays an important role in predicting response to various therapies and should be treated as a variable before starting any treatment. Therefore, more detailed research is needed in regions where HBV is hyperendemic. Key words: genotype, hepatitis B, hepatocellular carcinoma, liver enzymes, PCR, primers, viruses.