Volume : 11, Issue : 08, August – 2024
Title:
IN SILICO AND IN VITRO ANTI-INFLAMMATORY ACTIVITY OF ETHANOLIC LEAF EXTRACT OF BIOPHYTUM SENSITIVUM
Authors :
Harithahareendran.G*, Abhishek B Vijayan*, Arya.S.S*,Mrs. Anusree S, Mrs. Aswathy. S.S, Dr. Kiran K J, Dr. Prasobh GR
Abstract :
The present study aimed to evaluate the anti-inflammatory potential of ethanolic extract of Biophytum sensitivum leaves using in silico and in vitro methods. Preliminary phytochemical screening indicated the presence of flavonoids, phenolic compounds, essential oils, saponins. In silico studies are carried out using molecular docking studies, ligand molecule amentoflavone shows binding affinity with 1C7V and 1YPU receptors respectively. The extract inhibits protein denaturation, cyclooxygenase, lipoxygenase. In this study, the results demonstrated that the ethanolic extract of Biophytum sensitivum leaves possess moderate anti-inflammatory activity.
Keywords: Anti- inflammatory activity, Biophytum sensitivum, Docking studies, In vitro studies.
Cite This Article:
Please cite this article in press Harithahareendran.G et al In Silico And In Vitro Anti-Inflammatory Activity Of Ethanolic Leaf Extract Of Biophytum Sensitivum.,Indo Am. J. P. Sci, 2024; 11 (08).
Number of Downloads : 10
References:
1. Iwalewa EO, McGaw LJ, Naidoo V, Eloff JN. Inflammation: the foundation of diseases and disorders. A review of phytomedicines of South African origin used to treat pain and inflammatory conditions. J Ethnopharmacol. 2007;114(3):455-66.
2. Ferrero-Miliani L, Nielsen OH, Andersen PS, et al. Chronic inflammation: Importance of NOD2 and NALP3 in interleukin-1beta generation. Clin Exp Immunol. 2007;147:227–35.
3. Kaminska B. MAPK signaling pathways as molecular targets for anti-inflammatory therapy from molecular mechanisms to therapeutic benefits. Biochim Biophys Acta. 2005;1754:253–62.
4. Rhen T, Cidlowski JA. Anti-inflammatory action of glucocorticoids New mechanisms for old drugs. N Engl J Med. 2005;353:1711-23.
5. Sakthivel KM, Guruvayoorappan C. Biophytum sensitivum: Ancient medicine, modern targets. J Adv Pharm Technol Res. 2012;3(2):83-91.
6. Pawar AT, Vyawahare NS. Phytochemical and pharmacological profile of Biophytum sensitivum (L) DC. Int J Pharm Sci. 2014; 6(11):18-22.
7. Roman R, Pintilie L, Nuță D, Avram S, Buiu C, Sogor C, Limban C. In Silico Prediction, Characterization and Molecular Docking Studies on New Benzamide Derivatives. Processes. 2023;11(2):479.
8. Barh D, Chaitankar V, Yiannakopoulou EC, Salawu EO, Chowbina S, Ghosh P, Azevedo VA. In Silico Models: From Simple Networks to Complex Diseases. In: Animal Biotechnology.2014;385-403.
9. Guruvayoorappan C, Kuttan G. Immunomodulatory and Antitumor Activity of Biophytum sensitivum. Asian Pac J Cancer Prev. 2007;8:27-32.
10. Silva GO, Abeysundara AT, Aponso MM. Extraction methods, qualitative and quantitative techniques for screening of phytochemicals from plants. American Journal of Essential Oils and Natural Products. 2017; 5(2):29- 32.
11. Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and Extraction: A Review. Internationale Pharmaceutica Sciencia. 2011; 1(1):98- 106.
12. Muhammad SA, Fatima N. In silico analysis and molecular docking studies of potential angiotensin-converting enzyme inhibitor using quercetin glycosides. Pharmacogn Mag. 2015 Apr-Jun;11:S123-6
13. Mizushima Y and Kobayashi M. Interaction of anti-inflammatory drugs with sermproteins, especially with some biologically active proteins. Journal of Pharma Pharmacol. 1968; 20:169‐ 173.
14. Sakat S, Juvekar AR, Gambhire MN. In vitro antioxidant and anti-inflammatory activity of methanol extract of
15. Oxalis corniculata Linn. International Journal of Pharma and Pharmacological Sciences 2010; 2(1):146-155.
16. Gierse Walker MC, Gierse JK. In vitro assays for cyclooxygenase activity and inhibitor characterization. Methods Mol Biol. 644, 2010, 131-44.
17. Axelrod B, Cheesbrough TM, Laakso S. Lipoxygenase from soybeans. Methods Enzymol. 1981;71:441-450.